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Bacillus subtilis Pgrac01 Expression Vectors

Bacillus subtilis Pgrac01 Expression Vectors MoBiTec GmbH 2019 Page 2 MoBiTec GmbH, Germany Phone: +49 551 70722 0 Fax: +49 551 70722 22 E-Mail: Contents 1. Features .. 3 Recombinant protein production with Bacillus subtilis .. 3 Pgrac01 inducible Expression Vectors .. 3 2. Introduction .. 3 3. The Pgrac01 Expression Vectors .. 4 vector Map pHT01 .. 5 Location of tags in pHT01 derivatives .. 6 vector Map pHT43 .. 7 vector Map pHT1464 .. 8 4. Bacillus subtilis Host Strains .. 9 5. Storage and Handling Instructions .. 9 6. Growth Conditions .. 9 7. Transformation of Bacillus subtilis .. 10 Protocol A - Natural Competence.

vectors are structurally and segregationally stable, using the theta-mode of replication within B. subtilis (Jannière et al., 1990; Titok et al., 2003). Expression of the gene of interest is regulated by the Pgrac01 promoter fused to the lac operator allowing the induction by addition of ITPG. ...

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