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Bacillus subtilis Pgrac01 Expression Vectors

Bacillus subtilis Pgrac01 Expression Vectors MoBiTec GmbH 2019 Page 2 MoBiTec GmbH, Germany Phone: +49 551 70722 0 Fax: +49 551 70722 22 E-Mail: Contents 1. Features .. 3 Recombinant protein production with Bacillus subtilis .. 3 Pgrac01 inducible Expression Vectors .. 3 2. Introduction .. 3 3. The Pgrac01 Expression Vectors .. 4 Vector Map pHT01 .. 5 Location of tags in pHT01 derivatives .. 6 Vector Map pHT43 .. 7 Vector Map pHT1464 .. 8 4. Bacillus subtilis Host Strains .. 9 5. Storage and Handling Instructions .. 9 6. Growth Conditions .. 9 7. Transformation of Bacillus subtilis .. 10 Protocol A - Natural Competence .. 10 Protocol B - Electroporation .. 11 Media and Solutions .. 11 8. Induction with IPTG and Sample Analysis .. 12 Preparation of soluble and insoluble cell extracts from B. subtilis .. 12 Precipitation of proteins from culture supernatant.

commercially available enzymes are produced by Bacillus species. B. subtilis is advantageous in that it is non-pathogenic, does not have significant bias in codon usage, and is capable of secreting functional proteins directly into the culture medium in large scale. A large body of information is available concerning genome sequence, transcription,

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