Transcription of Bacteriology Washington - jb.asm.org
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Bacteriology Washington - jb.asm.org
,WashingtonUniversitySchoolofMedicine, ,MissouriReceivedforpublication,December 2,1935In1918 BronfenbrennerandDavisobservedthatintest inalbacteriawerefrequentlyencounteredwhi chfailedtoshowupaslactosefermentersinthe routinediagnosticprocedure, ,withthepurposeinviewofimprovingmethodsf ortheirdetection, (1906)andMassini(1907), (1934),Sandiford(1935),and,withspecialre ferencetobacterialvariation,byLewis(1934 )andHall(1935).Inthecourseofcontinuedstu diesofthemetabolismofslowlactose-ferment ingbacteriaoftheEscherichiacolitype, ( )indicator(Bronfen-brenner,1918).Ofsixsa mplesexamined,slowlactose-fer-mentingorg anismswereobtainedfromone, on February 26, 2021 by from , ,theyweresubjectedtodailyserialtransferi nalactosemediumasinthemethodprevi-ouslyu sed(BronfenbrennerandDavis,1918).Someela bora-tionsoftechnicwereintroducedtofacil itatethestudyofcondi-tionsaffectingthech angefromslowtorapidfermentation, ,oneseriesconsistingofopentubespluggedwi thcotton, ,onecultureineachserieswasusedtoinoculat ethesubcultures, ,transfersfromtheopentubecul-tureseriesw eremadeintosealedfermentationtubestoobse rveanychangesinfermentationreactions, ,correspondingtosubcultureIofeachseries( table1).
Department of Bacteriology and Immunology, Washington University School of Medicine, St. Louis, Missouri Receivedfor publication, December2, 1935 In 1918 Bronfenbrenner and Davis observed that intestinal bacteriawerefrequently encounteredwhichfailed to showupas
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