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BASIC PRACTICAL MICROBIOLOGY

BASIC PRACTICAL MICROBIOLOGY A MANUAL Compiled by John Grainger, Chairman of MISAC Janet Hurst, Deputy Executive Secretary of SGM Dariel Burdass, SGM Educational Projects Administrator BASIC PRACTICAL MICROBIOLOGY : A MANUAL The Society for General MICROBIOLOGY 2001 Contents Part 1: THE basics An introduction to MICROBIOLOGY , aseptic technique and safety Page Number PREPARATION Safety guidelines 1 Risk assessment 1 Good microbiological laboratory practice (GMLP) 3 Spillage management 3 Aerosols 4 RESOURCES Equipment 4 Apparatus 5 Materials 5 MEDIA, STERILIZATION AND DISINFECTANTS Preparation of culture media 6 Storage of media 6 Sterilization vs.

Level of practical work (Level 1, Level 2, Level 3, Topics in Safety, 3rd edition (ASE, 2001), Topic 15) or Factsheet: Safety ... Sterilization of metal forceps and glass spreaders by ignition Autoclave indicator tape Changes colour in response to heat to distinguish those items that .

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