Transcription of BASIC PRACTICAL MICROBIOLOGY
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BASIC PRACTICAL MICROBIOLOGY
BASIC PRACTICAL MICROBIOLOGY A MANUAL Compiled by John Grainger, Chairman of MISAC Janet Hurst, Deputy Executive Secretary of SGM Dariel Burdass, SGM Educational Projects Administrator BASIC PRACTICAL MICROBIOLOGY : A MANUAL The Society for General MICROBIOLOGY 2001 Contents Part 1: THE basics An introduction to MICROBIOLOGY , aseptic technique and safety Page Number PREPARATION Safety guidelines 1 Risk assessment 1 Good microbiological laboratory practice (GMLP) 3 Spillage management 3 Aerosols 4 RESOURCES Equipment 4 Apparatus 5 Materials 5 MEDIA, STERILIZATION AND DISINFECTANTS Preparation of culture media 6 Storage of media
instead of in liquid media whenever possible. Care should also be taken to avoid generating aerosols during practical work. The risk is minimized by adhering to GMLP with special attention to the correct use of pipettes (see “Inoculation and other aseptic procedures” page 8). RESOURCES Equipment Equipment Use Loop (wire/plastic)
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