Basic Principles of Cell Culture - Universidad de Granada

1 Basic Principles of Cell CultureR. Ian FreshneyCentre for Oncology and Applied Pharmacology, Cancer Research UK BeatsonLaboratories, Garscube Estate, Bearsden, Glasgow G61 1BD, Scotland, types of Cell Primary Explantation Versus Proliferation Versus Organotypic Substrates and Isolation of cells for tissue Collection and Biosafety and Record Disaggregation and Primary Life Growth Serial Characterization and Microbial of of cells for tissue Engineering, edited by Gordana Vunjak-Novakovic and R. Ian FreshneyCopyright 2006 John Wiley & Sons, 1. INTRODUCTIONThe bulk of the material presented in this book assumes background knowledgeof the Principles and Basic procedures of cell and tissue Culture . However, it isrecognized that people enter a specializedfield, such as tissue engineering, frommany different disciplines and, for this reason, may not have had any formaltraining in cell Culture . The objective of this chapter is to highlight those prin-ciples and procedures that have particularrelevance to the use of cell culturein tissue engineering.

epithelial layer of the epidermis, that gives rise to the bulk of the cells in a 4 Chapter 1. Freshney. ... of all the participating cell types in the tissue in vivo and the ability to culture ... are termed histotypic cultures and aim to attain the density of cells found in the tissue from which the cells were derived (Fig. 1.4). It is ...

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  Types, Tissue, Culture, Epithelial, Cells, Found, Cell culture

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