Transcription of Bead Based Assays for Cytokine Detection - BNITM
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Bead Based Assays for Cytokine Detection - BNITM
Bead Based Assays for Cytokine Detection September 27, 2014 6th EFIS-EJI South East European Immunology School SEEIS 2014 Timisoara, Romania The Cells of the Immune System The Immune Reaction (Th2) (Th1) The Immune Reaction T cell B cell Mast cell Neutrophil Basophil Eosinophil Macrophage IL-2, 4, 6, 8, 9, IFNa, b, g IL-1, 6, 8, 10, 12, 15, IFNa, b, TNFa IL-5 IL-4 IL-10 IL-1, 2, 4, 6, 10, 13, 14, IFNg, TNFa, b IL-1, 3, 4, 10, 13, IFNa, b, g, TNFa, b IL-1, 2, 4, 6, 8, 9, IFNa, b, g IL-10, 12, TNFa, b IL-3, 4, 5 IL-4 IFNa, b, TNFa IL-4 IL-1, IFNa, b, TNFa, b IL-1, 6, 10, 12, IFNa, b, TNFa, b Applications for Monitoring the Immune Functions Tools & Technologies for Immune Function Monitoring Applications for Monitoring the Immune Functions Surface & Intracellular Analysis by Flow Cytometry Block Fc Rceptors & Cell Surface Staining Anti IFN g CD69 Principle of Cell Surface Staining CD8 Principle of Intracellular Cytokine Staining (ICS)
Bead Based Assays for Cytokine Detection September 27, 2014 6 ... –Cytometric: Data acquired on a flow cytometer is analysed using specific software –Bead: The system is composed of various beads with different fluorescence intensity coated with high affinity capture antibodies –Array: Technology allowing multiplex analysis CBA - …
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