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Bergey’s Manual of Determinative Bacteriology

Identification flow charts 1 Bergey s Manual of Determinative Bacteriology All of the unknowns will fall into the following groups in Bergey's Manual of Determinative Bacteriology (The pink book on the shelf in the laboratory). GROUP 4 Description: Gram Negative, Aerobic/Microaerophilic rods and cocci Key differences are: pigments/fluorescent, motility, growth requirements, denitrification, morphology, and oxidase, read Genera descriptions Examples: Acinetobacter, Pseudomonas, Beijerinckia, Acetobacter GROUP 5 Description: Facultatively Anaerobic Gram negative rods Key differences are: growth factors, morph.

Bile Esculin (growth with esculin hydrolysis) Growth w/ Tellurite Hemolysis Optochin Sensitivity Pyrrolidonearylamidase (PYR Test) Yes No +-+ + + + + + Yes No-----Gamma (!) Beta (") Alpha (#) Identification flow charts 6 Gram Negative Rods ID Flowchart Aeromonas, Pseudomonas, Vibrio Enterobacteriaceae

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