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Bergey’s Manual of Determinative Bacteriology

Identification flow charts 1 Bergey s Manual of Determinative Bacteriology All of the unknowns will fall into the following groups in Bergey's Manual of Determinative Bacteriology (The pink book on the shelf in the laboratory). GROUP 4 Description: Gram Negative, Aerobic/Microaerophilic rods and cocci Key differences are: pigments/fluorescent, motility, growth requirements, denitrification, morphology, and oxidase, read Genera descriptions Examples: Acinetobacter, Pseudomonas, Beijerinckia, Acetobacter GROUP 5 Description: Facultatively Anaerobic Gram negative rods Key differences are: growth factors, morph., gram rxn., oxidase rxn., read Genera descriptions Examples: Family Enterobacteriaceae and Vibrionaceae GROUP 17 Description: Gram-Positive Cocci Key differences are: oxygen requirements, morph.

Lactobacillus delbrueckii Lactobacillus casei Lactobacillus fermenti Gram Positive Rods Spore Forming Acid Fast Strict Anaerobes Catalase Starch Hydrolysis Glucose Ferm. Activity Acid & Gas Acid Mannitol + -----+ + + + + Identification flow charts 4 Bacillus spp. ID Flowchart B. subtilis B. cereus B. megaterium

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