Transcription of BIOL1414 Lab Manual Fall 2011 - Austin Community College ...
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BIOL1414 Lab Manual Fall 2011 - Austin Community College ...
BIOL1414 Lab Manual Fall 2011 1 Austin Community College , Biotechnology Department Introduction to Biotechnology Fall 2011 Linnea Fletcher, Evelyn Goss, Patricia Phelps, Angela Wheeler, and Shelley O Grady ISBN: BIOL1414F2011 BIOL 1414 Laboratory Manual BIOL1414 Lab Manual Fall 2011 2 BIOL1414 Lab Manual Fall 2011 3 Table of Contents LAB UNIT 1: INTRODUCTION TO BIOL1414 LAB .. 5 Lab Unit 1-A: Documentation: The Lab Notebook & Lab Report .. 11 Lab Unit 1-B: Biotechnology Laboratory Security & Safety .. 17 Lab Unit 1-C: Lab Equipment & Reagent Orientation .. 25 Lab Unit 1-D: Math Skills for the Laboratory .. 31 LAB UNIT 2: CURRENT GOOD MANUFACTURING PRACTICES (cGMP) .. 39 LAB UNIT 3: BASIC TOOLS IN THE BIOTECHNOLOGY LABORATORY .. 85 Lab Unit 3-A: Using a 91 Lab Unit 3-B: Calibrating Lab Instruments .. 97 LAB UNIT 4: PREPARING SOLUTIONS .. 103 LAB UNIT 5: TOTAL RNA ISOLATION FROM ALFALFA SPROUTS .. 115 Lab Unit 5-A: Total RNA Isolation .. 121 Lab Unit 5-B: Denaturing Gel Electrophoresis of Total RNA.
BIOL1414 Lab Manual Fall 2011 6 What is biotechnology? Strictly speaking, biotechnology is the use of a living organism for one‘s own benefit. By this definition, biotechnology would date back to the very beginnings of civilization, when humankind first learned to cultivate crops and domesticate animals in a system of agriculture.
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