Biological Reactors (Chemostat) - MIT OpenCourseWare

Chemical and Biological Reaction Engineering, Spring 2007 Prof. K. Dane Wittrup lecture 13: Biological Reactors - Chemostats This lecture covers: theory of the chemostat , fed batch or semi-continuous fermentoroperations Biological Reactors ( chemostat ) Concentration/Combustion constant Biological CSTR [S]0 V F F [S], x Figure 1. Diagram of a chemostat . F = Volumetric flow rate biomassx= volume [S]0 = Concentration of growth limiting substrate. (for growing cells) At steady-state, biomass balance In Out + Prod = Acc Sterile feed: In=0 Steady state: Acc=0 +Fx rV0 at steady-state x=Cell growth kinetics rxx= +Fx xV0= Solve F = VD=Dilution rateF1 = V =DBiological Mechanical Cite as: K. Dane Wittrup, course materials for Chemical and Biological Reaction Engineering, Spring 2007. MIT OpenCourseWare ( ), Massachusetts Institute of Technology. Downloaded on [DD Month YYYY]. When at steady-state, can control cell mass.

For real systems KSs <<[]0. Most cell growth systems reach maximum at fairly low concentrations; hence x is flat, then drops off sharply. biomass is the product, is there a best operating condition? hat should we consider? 10.37 Chemical and Biological Reaction Engineering, Spring 2007 Lecture 13 Prof. K. Dane Wittrup Page 3 of 6

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