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Biological Reactors (Chemostat) - MIT OpenCourseWare

chemical and Biological Reaction engineering , Spring 2007 Prof. K. Dane Wittrup Lecture 13: Biological Reactors - Chemostats This lecture covers: theory of the chemostat , fed batch or semi-continuous fermentoroperations Biological Reactors ( chemostat ) Concentration/Combustion constant Biological CSTR [S]0 V F F [S], x Figure 1. Diagram of a chemostat . F = Volumetric flow rate biomassx= volume [S]0 = Concentration of growth limiting substrate. (for growing cells) At steady-state, biomass balance In Out + Prod = Acc Sterile feed: In=0 Steady state: Acc=0 +Fx rV0 at steady-state x=Cell growth kinetics rxx= +Fx xV0= Solve F = VD=Dilution rateF1 = V =DBiological Mechanical Cite as: K. Dane Wittrup, course materials for chemical and Biological Reaction engineering , Spring 2007. MIT OpenCourseWare ( ), Massachusetts Institute of Technology.

ogistic equation” “L x t Figure 5. Graph of logistic growth. If product is something cells are making: Product synthesis kinetics 1) 10.37 Chemical and Biological Reaction Engineering, Spring 2007 Lecture 13

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