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CALIBRATION OF MICROSCOPE EYEPIECE GRATICULE

CALIBRATION OF MICROSCOPE EYEPIECE GRATICULE A typical EYEPIECE GRATICULE looks like this: It is 10mm in length and each mm is divided into 10 parts So each small division = = 100 m The EYEPIECE GRATICULE is located at the primary image of the MICROSCOPE . So it is in focus with image of the specimen. The specimen is magnified onto the GRATICULE by the magnification of the objective. So, the actual amount of specimen superimposed on the GRATICULE is: Objective magnification Typically this gives the following calibrations (with an EYEPIECE GRATICULE 10mm in 100 parts): Objective Magnification Each small division Equals (in mm) Each small division Equals in microns ( m) 4x 25 10x 10 20x 5 40x 100x 1 TO CALIBRATE HOWEVER There may be small differences between one MICROSCOPE and another.

CALIBRATION OF MICROSCOPE EYEPIECE GRATICULE A typical eyepiece graticule looks like this: It is 10mm in length and each mm is divided into 10 parts

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  Calibration, Microscope, Calibration of microscope

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