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流式细胞术的原理与应用 - CAS

1 FACS: fluorescence-activated cell sorting 2 FACS Aria 3 4 5 10 / 99% 6 BD FACS Aria 7 8 488nm ,633nm 405nm 375nm 561nm 9 407nm 633nm 488nm 10 460 500 540 460 500 540 460 500 540 SP 500 LP 500 BP 500/50 Longpass(LP) Shortpass(SP) Bandpass(BP) 11 PMT 12 FACS Aria BP LP 13 BD FACS Aria 14 PBS 15 16 1.

Annexin V/PI双染色法 磷脂酰丝氨酸(PS):凋亡早 期细胞的PS可从细胞膜的内侧 翻转到细胞膜的表面,暴露在 细胞外环境中。 Annexin-V:一种Ca2+依赖性磷 脂结合蛋白,能与PS高亲和力 特异性结合。将Annexin-V进行 荧光素标记,检测细胞凋亡的 发生。但不能区分凋亡 ...

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