Transcription of 共聚焦显微成像技术及图像处理 - CAS
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共聚焦显微成像技术及图像处理 - CAS
2020 8 7 Leica DLS Leica SP8 Zeiss LSM 710 NLO Leica STED Rat Brain, Double labelling: Green: Neurons, Blue: Nuclei PMT Laser Rat Brain, Double labelling: Green: Neurons, Blue: Nuclei Pinhole Laser Sample PMT Pinhole XY scanning Z-Drive d NA Laser 405nm 458nm/488nm/514nm 561nm 594nm 633nm Laser 405nm 448nm 488nm 552nm 638nm Laser 405nm 458nm/476nm/488nm/496nm/514nm WIL 470nm-670nm Laser 690nm-1064nm 458nm/488nm/514nm 561nm 633nm 90 PMT PMT 1 2 3
普通共聚焦成像及frap、fret、光转化、光激活等特殊成像 用于测定荧光强度、共定位、三维重构和蛋白动力学数据和蛋白相互作用等结果 双光子共聚焦成像 利用双光子激发化学合成小分子纳米材料标记目标蛋白，或进行厚样品的深度观察
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