CELL CULTURE BASICS - Vanderbilt University

CELL CULTURE BASICS . Cell CULTURE BASICS Handbook B-087243 0110. Contents Introduction .. 1. Purpose of the Handbook .. 1. Introduction to Cell CULTURE .. 2. What is Cell CULTURE ? .. 2. Finite vs Continuous Cell Line .. 2. CULTURE Conditions .. 2. Cryopreservation .. 2. Morphology of cells in CULTURE .. 3. Applications of Cell CULTURE .. 3. Cell CULTURE Laboratory .. 4. Safety .. 4. Biosafety Levels .. 4. SDS .. 5. Safety Equipment .. 5. Personal Protective Equipment (PPE) .. 5. Safe Laboratory Practices .. 5. Cell CULTURE Equipment .. 6. Basic Equipment .. 6. Expanded Equipment .. 6. Additional Supplies .. 6. Cell CULTURE Laboratory .. 7. Aseptic Work Area .. 7. Cell CULTURE Hood .. 7. Cell CULTURE Hood Layout .. 8. Incubator .. 9. Storage .. 9. Cryogenic Storage .. 10. Cell Counter .. 10. Cell CULTURE BASICS | i Contents Aseptic Technique .. 11. Introduction .. 11. Sterile Work Area .. 11. Good Personal Hygiene.

Mar 09, 2015 · severity by ingestion or through percutaneous or mucous membrane exposure. Most cell culture labs should be at least BSL-2, but the exact requirements depend upon the cell line used and the type of work conducted. Biosafety Level 3 (BSL-3) BSL-3 is appropriate for indigenous or exotic agents with a known potential for aerosol

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