Transcription of Champion pET SUMO Protein Expression System
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Champion pET SUMO Protein Expression System
User Manual Champion pET SUMO Protein Expression System For high-level Expression and enhanced solubility of recombinant proteins in E. coli and cleavage of native Protein Catalog no. K300-01 Rev. Date: 18 June 2010 Manual part no. 25-0709 MAN0000440 ii iii Table of Contents Kit Contents and Storage ..v Accessory T7-Regulated Expression ..3 Experimental Outline ..5 Methods .. 6 Cloning Considerations ..6 Producing PCR Products ..8 Cloning into pET Transforming One Shot Mach1 -T1R Competent Cells ..10 Analyzing Expressing the PCR Product ..13 Analyzing Samples ..16 Purifying the Recombinant Fusion Using SUMO Troubleshooting ..22 Performing the Control Reactions.
Product Quantity Catalog no. ProBond™ Nickel-Chelating Resin 50 ml R801-01 150 ml R801-15 ProBond™ Purification System 6 purifications K850-01 Ni-NTA Agarose 10 ml R901-01 25 ml R901-15 100 ml R901-10 Ni-NTA Purification System 6 purifications K950-01 Polypropylene Columns (empty) 50 R640-50
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