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CHAPTER 1 Introduction, Chromatography Theory, and ...

1 CHAPTER 1 Introduction, Chromatography Theory, and Instrument Calibration Introduction Analytical chemists have few tools as powerful as Chromatography to measure distinct analytes in complex samples. The power of Chromatography comes from its ability to separate a mixture of compounds, or analytes , and determine their respective identity (chemical structure) and concentration. Chromatography can be divided into three basic types that include gas, liquid, and supercritical fluid Chromatography . Liquid Chromatography can further be divided into ion exchange, separations based on size, and even extended to gel-based electrophoretic techniques. This book will provide a basic introduction to different types of liquid and gas Chromatography . The relationship between each type of Chromatography is illustrated in Figure Figure Categories of Chromatography and Their Relationship to Each Other. 2 In general, each type of Chromatography is comprised of two distinct steps: Chromatography (or separation of individual compounds in distinct elution bands) and identification (detection of each elution band).

based electrophoretic techniques. This book will provide a basic introduction to different types of liquid and gas chromatography. The relationship between each type of chromatography is illustrated in Figure 1.1. Figure 1.1. Categories of Chromatography and Their Relationship to …

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