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CHAPTER 1 Introduction, Chromatography Theory, and ...

1 CHAPTER 1 introduction , Chromatography Theory, and Instrument Calibration introduction Analytical chemists have few tools as powerful as Chromatography to measure distinct analytes in complex samples. The power of Chromatography comes from its ability to separate a mixture of compounds, or analytes , and determine their respective identity (chemical structure) and concentration. Chromatography can be divided into three basic types that include gas, liquid, and supercritical fluid Chromatography . Liquid Chromatography can further be divided into ion exchange, separations based on size, and even extended to gel-based electrophoretic techniques. This book will provide a basic introduction to different types of liquid and gas Chromatography .

structure of the analyte. Principles of gas chromatography (GC) will be covered in Chapter 2, liquid chromatography (LC) in Chapter 3, capillary electrophoresis (CE) in Chapter 4 and mass spectrometry (MS) in Chapter 5. In mass spectrometry, …

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