Transcription of Chapter 1 INTRODUCTION TO NMR SPECTROSCOPY
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Chapter 1 INTRODUCTION TO NMR SPECTROSCOPY
Chapter 1. INTRODUCTION TO NMR SPECTROSCOPY . INTRODUCTION Nuclear magnetic resonance (NMR) is a spec- troscopic technique that detects the energy ab- sorbed by changes in the nuclear spin state. The application of NMR SPECTROSCOPY to the study of proteins and nucleic acids has provided unique in- formation on the dynamics and chemical kinetics of these systems. One important feature of NMR. is that it provides information, at the atomic level, on the dynamics of proteins and nucleic acids over an exceptionally wide range of time scales, ranging from seconds to pico-seconds. In addition, NMR. can also provide atomic level structural informa- tion of proteins and nucleic acids in solution (see Fig.)
4 Introduction to NMR Spectroscopy Table 1.2. Properties of NMR Active Nuclei. Nuclei1 γ(rad·sec−1 · gauss−1)† INaturalAbundance(%) 1H26,753 1/2 99.980 2H4,106 1 0.016 19F25,179 1/2 100.0002 13C6,728 1/2 1.1083 31P10,841 1/2 100.00 1The term “Protons” is used interchangeably with 1Hinthetext. 2Fluorine is not normally found in biopolymers, therefore it …
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