Transcription of CHAPTER 11 Mechanism of Enzyme Action
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CHAPTER 11 Mechanism of Enzyme Action 1. General properties of enzymes 2. Activation energy and the reaction coordinate 3. Catalytic Mechanism 4. Lysozyme 5. Serine proteases Enzyme act with great speed and precision Introduction 1. Enormous variety of chemical reactions within a cell 2. Mediated by Enzymes 3. Enzymology, the study of enzymes (coined 1878; Greek: en, in; zyme, yeast), fermentation: glucose -> ethanol 12 Enzyme -catalyzed steps 4. James Summer, 1926, crystallized urease from jack bean, shown to be a protein 5. Other catalysts, ribozymes (peptide-bond formation; RNA-world ), only for units 6. Proteins more versatile, 20 functional units Introduction Enzymes increase the rate of chemical reactions by lowering the free energy barrier that separates the reactants and products 1.
Enormous variety of chemical reactions within a cell 2. Mediated by Enzymes 3. ... nucleophilic group on enzyme attacks an electrophilic group on the substrate ... Stabilization through H bridges and ionic interactions . The interactions of lysozyme
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