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Chapter 14

At the end of this lab, students will be able to: discuss the principles that govern protein separation on discontinuous SDS-PAGE gels. cast and run SDS-PAGE gels. analyze the pattern of bands on a stained SDS-PAGE gel estimate the molecular weight of a protein from its migration on SDS-PAGE gelsThis lab will introduce you to SDS-PAGE, a simple and inexpensive method for resolving proteins in complex mixtures. SDS-PAGE gels provide the starting materials for western blots and for some proteomic techniques. In this lab, you will use SDS-PAGE to analyze the protein extracts that you prepared from yeast strains overexpressing Met and LacZ fusion proteins. SDS-PAGEC hapter 14 Objectives124 Chapter 14 SDS-PAGE is widely used to analyze the proteins in complex extracts.

Chapter 14 Objectives. 124 Chapter 14 SDS-PAGE is widely used to analyze the proteins in complex extracts. The most ... any covalent bonds between cysteine residues. Like other detergents, SDS is an amphipathic ... is 9.6, considerably higher than the …

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