Transcription of Chapter 4 –Microscopy - Quantitative Light Imaging ...
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Chapter 4 –Microscopy - Quantitative Light Imaging ...
Chapter 4 Microscopy Gabriel PopescuUniversity of Illinois at Urbana ChampaignypgBeckman InstituteQuantitativeLightImagingLaborat oryElectrical and Computer Engineering, UIUCP rinciples of Optical ImagingQuantitative Light Imaging 460 Optical Imaging The Microscope can be approximated by 2 lenses [ [ [ , ]]] Resolution of Optical MicroscopespppyTube lensSampleF2 F2 Objective[ [ [ , ]]]yU(x,y)F 1[[ ]]FUxy[[[ ]]] []xyFFUU sign means inverted image Theobjectiveisthemostimportantpartofthem icroscope1[[,]]FUxy21[[[,]]][,]yFFUxyUMM The objective is the most important part of the microscope Usually a third lens (ocular) images F2 at , such that we can visualize it with the relaxed 4: Microscopy 460 Optical Imaging The objective lens dictates the res
ECE 460 –Optical Imaging 4.2 Contrast No absorption so I[, ]x y constant contrast = 0 BUT:the wave front carries information about the sample Exy E e[] ixy [, ] (4.14 ) This is the expression for the field in the vicinity of a phase object [ , ] 0.
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