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Chapter 5 - Bacteriology - FWS

NWFHS Laboratory Procedures Manual - Second Edition, June 2004 Chapter 5 - Page 1 Chapter 5 Bacteriology Jason Woodland USFWS - Pinetop Fish Health Center Pinetop, Arizona NWFHS Laboratory Procedures Manual - Second Edition, June 2004 Chapter 5 - Page 2 I. Introduction This section defines the procedures and techniques used to correctly identify the target bacterial pathogens identified for the Survey (Yersinia ruckeri, Aeromonas salmonicida and Edwardsiella ictaluri). Proper identification relies on bacterial growth characteristics, appropriate biochemical tests, and corroboration by serological techniques. Pathogens of Regional Importance (PRIs) include: Citrobacter freundii, Edwardsiella tarda, Flavobacterium columnare and Flavobacterium psychrophilum. The later two bacteria have special requirements for culture and serological confirmation.

refrigerated for later use. Store bottles labeled with media type, date and initials. When media is needed, boil, microwave or use a water bath to completely melt the agar. Cool to 50°C, then proceed to step 5. 5. Before pouring media, disinfect hood or counter thoroughly and place sterile petri dishes on the disinfected surface. 6.

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