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Chapter 5 - Bacteriology - FWS

NWFHS Laboratory Procedures Manual - Second Edition, June 2004 Chapter 5 - Page 1 Chapter 5 Bacteriology Jason Woodland USFWS - Pinetop Fish Health Center Pinetop, Arizona NWFHS Laboratory Procedures Manual - Second Edition, June 2004 Chapter 5 - Page 2 I. Introduction This section defines the procedures and techniques used to correctly identify the target bacterial pathogens identified for the Survey (Yersinia ruckeri, Aeromonas salmonicida and Edwardsiella ictaluri). Proper identification relies on bacterial growth characteristics, appropriate biochemical tests, and corroboration by serological techniques.

2. Gram positive: cells retain the crystal violet and remain purple to dark blue. K. QUALITY CONTROL: Use a known bacterial culture as a control for each case history to assess correct staining and interpretation (cultures can be obtained from ATCC® and maintained at 2-8°C for long term use). Additionally Fisher Scientific has gram control

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