Transcription of Chapter 6: Laboratory Identification of Vibrio cholerae
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Chapter 6: Laboratory Identification of Vibrio cholerae
Laboratory Methods for the Diagnosis of Vibrio cholerae Centers for Disease Control and Prevention 38 | Page Laboratory Methods for the Diagnosis of Vibrio cholerae Centers for Disease Control and Prevention VI. Laboratory Identification OF Vibrio cholerae Members of the genus Vibrio are facultatively anaerobic, asporogenous, motile, curved or straight gram-negative rods. Vibrios either require NaCl or have their growth stimulated by its addition. All members of the genus Vibrio , with the exceptions of V. metschnikovii and V. gazogenes, are oxidase positive and reduce nitrates to nitrites.
Isolation of V. cholerae non-O1 from ... to remove a portion of the growth from the surface of heart infusion agar (HIA), Kligler’s iron agar (KIA), triple sugar iron agar (TSI), or other nonselective agar medium. Emulsify the growth ... b Nutrient broth base (Difco Laboratories) c. Most isolates of . V. cholerae.
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