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college, validate it on sub-sets of the 1000 students that were not included when creating the prediction model, and then use the model to make predictions about new students.

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analysis, or fluorescence microscopy. If immobilized cells on coverslips are needed, use poly-D-lysine to coat the slides or coverslips before mounting. If the cells are to be fixed and permeabil ized, continue to Fixation and Permeabilization after Staining. Optional: Fixation and Permea-

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microscopy of two consecutive sputum specimens will identify the vast majority (95–98%) of smear-positive TB patients**. Moreover, microscopy can be decentralised to peripheral laboratories. Despite its advantages sputum smear microscopy does fall short in test sensitivity, especially for certain patient groups such as those living

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