Transcription of Chem 321 Lecture 6 - Calibration Methods
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Chem 321 Lecture 6 - Calibration Methods
Chem 321 Lecture 6 - Calibration Methods9/12/13 Student Learning ObjectivesCalibration MethodsMost analytical Methods rely on a standard. Such an approach is sometimesreferred to as a comparator method. In such a procedure, a carefully preparedstandard is run through the same analysis procedure used for the unknown samplesand the results (signals) from the standard and unknown are compared. When manystandards are run, a Calibration curve is usually made in which the signal from thestandard analysis is plotted against the standard concentration. Often the relationshipbetween the signal and concentration is linear ( , the signal is directly proportional toconcentration) over some range of concentrations and a linear least-squares line isused to fit the data.
Calibration Methods 9/12/13 page 5 The confidence interval for x is then given by ±tsx where t corresponds to the value for n - 2 degrees of freedom. This rather daunting formula for sx is rather easily evaluated using Excel and the LINEST function.
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