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Chem 321 Lecture 6 - Calibration Methods

Chem 321 Lecture 6 - Calibration Methods9/12/13 Student Learning ObjectivesCalibration MethodsMost analytical Methods rely on a standard. Such an approach is sometimesreferred to as a comparator method. In such a procedure, a carefully preparedstandard is run through the same analysis procedure used for the unknown samplesand the results (signals) from the standard and unknown are compared. When manystandards are run, a Calibration curve is usually made in which the signal from thestandard analysis is plotted against the standard concentration. Often the relationshipbetween the signal and concentration is linear ( , the signal is directly proportional toconcentration) over some range of concentrations and a linear least-squares line isused to fit the data.

Calibration Methods 9/12/13 page 6 Example 3.1 Problem Determine the uncertainty in the vitamin B2 concentration of an unknown based on the data below and find …

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