Transcription of ChromoCult Coliform Agar
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ChromoCult Coliform Agar
ChromoCult Coliform AgarFor detection of coliforms in drinkingwater and processed food of actionPrimarily the interaction of carefully selected peptones inaddition to pyruvate, sorbitol and a phosphate bufferguarantees rapid colony growth, even for the sublethallyinjured coliforms. The growth of accompanying Gram-positive bacteria flora aswell as some Gram-negative bacteria flora is largely inhibitedby the use of Tergitol 7 which has no negative effect on thegrowth of the Coliform , Merck has developed a new combination of two chromogenic substrates which allow for the simultaneousdetection of total coliforms and identificationThe characteristic enzyme for coliforms, -D-galactosidase,cleaves the Salmon-GAL substrate and resulting in a salmonto red colouration of the Coliform identificationThe substrate X-glucuronide is used for the identification of -D-glucuronidase which is characteristic for cleaves both Salmon-GAL and X-glucuronide, so thatpositive colonies take on a dark-blue to violet colour.
Mode of action Primarily the interaction of carefully selected peptones in addition to pyruvate, sorbitol and a phosphate buffer guarantees rapid colony growth, even for the sublethally
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