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<< COPY >>B E A D S A B O V E T H E R E S T TechNote 205 Covalent Coupling9025 Technology Dr. Fishers, IN 46038-2886 Fax IntroductionII. Binding Protocol Design and Optimization A. Reactive Groups and coupling Chemistry B. Microsphere Composition C. Reagent Quality D. Reagent Concentration E. Buffers F. Blockers G. Bead Handling H. OtherIII. Sample coupling Protocols A. Carboxyl-Modified Microspheres B. Amino-Modified Microspheres C. Hydroxyl-Modified Microspheres D. Hydrazide-Modified Microspheres E. Chloromethyl-Modified MicrospheresIV. Miscellaneous coupling Strategies A. coupling to Non-Functionalized Polymeric Microspheres B. coupling to Non-Functionalized Silica Microspheres C. Conversion of Surface Functional Groups D. Covalent Attachment of Small MoleculesV. Evaluation of the Coating ProcedureVI.
<< COPY >> B E A D S A B O V E T H E R E S T ™ TechNote 205 Covalent Coupling 9025 Technology Dr. • Fishers, IN 46038-2886
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