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CONTENTS . Chapter Name of the Chapter Page No. Preface (i). Acknowledgment (ii). List of contributors (iii). Chapter 1 Introduction 1-2. Introduction 1. dengue in India 1-2. Chapter 2 Epidemiology 3-6. Epidemiology 3. Agent Factor 3. Vector 3-4. Environmental Factor 4-5. Host Factor 6. Transmission cycle 6. Chapter 3 Clinical Diagnosis of DHF/DSS 7-9. Immuno-pathogenesis 7. Clinical manifestation of DF/DHF 8-9. Chapter 4 Laboratory Diagnosis of dengue 10-13. Laboratory Diagnosis 10. Isolation of dengue Virus 10. Serological Tests 10. Haemagglutination inhibition (HI) test 11. Compliment Fixation Test (CFT) 11. Neutralization test (NT) 11. IgM Capture ELISA (Mac ELISA) 11-12. IgG ELISA 12. Rapid Diagnosis Tests (RDTs) 12. Collection of Specimens 12-13.

i PREFACE Dengue Fever/Dengue Haemorrhagic Fever is an acute viral disease having the potential of causing, large scale outbreaks. The risk of dengue has shown an increase in recent years

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