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Culture Media by Shyamal - Mymensingh Medical College

Dr Shyamal Kr Paul Associate Prof, Microbiology MMC. Culture Media . 12/30/13 Dr. Shyamal Kr Paul, Culture Media 1. Essential requirements in Culture Media Any Culture medium must contains: -A source of energy -Sources of carbon, nitrogen, sulfur, phosphorus -Minerals, , Ca2+, Mg2+, Na+. -Vitamins and growth factors - Water 12/30/13 Dr. Shyamal Kr Paul, Culture Media 2. Culture Media Culture Medium: Nutrients prepared for microbial growth Inoculum: Suspension of microorganisms Inoculation: Introduction of microbes into Culture medium Culture : Microbes growing in/on Culture medium A pure Culture contains only one species or strain Mixed Culture contains several species Contaminated Culture contains unwanted species of organisms 12/30/13 Dr. Shyamal Kr Paul, Culture Media 3.

Selective media: with inhibitory substances Differential media: with indicators/dyes Special Media for Biochemical Testing Media for Antimicrobial Susceptibility Testing 12/30/13 Dr. Shyamal Kr Paul, Culture media 15. Basic media These are media which may be used for

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– solubility in water or alcohol, stable – broad spectrum, low toxicity – penetrating – noncorrosive and nonstaining – affordable and readily available 12/30/13 Dr. Shyamal Kr Paul, Streilization. Levels of Chemical Decontamination • High-level germicides – kill endospores; may be

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