Transcription of Determination of Water- and Fat-Soluble Vitamins by HPLC
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Determination of Water- and Fat-Soluble Vitamins by HPLC
Determination of water- and Fat-Soluble Vitamins by HPLCTECHNICAL NOTE 72488 IntroductionVitamins are a well-known group of compounds that are essential for human health and are classified into two main groups, Water- soluble and Fat-Soluble . Water- soluble Vitamins include B group Vitamins (thiamine/B1, riboflavin/B2, nicotinamide/nicotinic acid/B3, pantothenic acid/B5, pyridoxine/pyridoxal hydrochloride/B6, folic acid/B9, and cyanocobalamine/B12) and ascorbic acid (vitamin C). Fat-Soluble Vitamins include retinol (vitamin A), tocopherol (vitamin E), calciferol (vitamin D), and antihemorrhagic Vitamins (vitamin K). These Vitamins play specific and vital functions in metabolism, and their lack or excess can cause health problems.
phosphate, formic acid, and acetic acid. Non-aqueous reversed-phase (NARP) retention is commonly used for fat-soluble vitamins so that the vitamins are soluble throughout the analysis. A typical NARP mobile phase consists of a polar solvent (acetonitrile), a solvent with lower polarity (e.g., dichloromethane) to act as
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