Transcription of Diafiltration for Desalting or Buffer Exchange
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Diafiltration for Desalting or Buffer Exchange
MAY2003 BioProcess International43 DDiafiltration is anultrafiltrationmembrane techniquefor completelyremoving, replacing, orlowering the concentration of saltsor solvents from solutionscontaining proteins, peptides,nucleic acids, and otherbiomolecules. The processselectively uses permeable (porous)membrane filters to separate thecomponents of solutions andsuspensions based on theirmolecular size. Smaller moleculessuch as salts, solvents, and waterpass freely through theultrafiltration membrane, whichretains the larger molecules. CONCENTRATIONThe solution retained by amembrane is known as concentrateor retentate.
MAY 2003 BioProcess International 47 removal. DISCONTINUOUS DIAFILTRATION Sequential Dilution. Discontinuous diafiltration by sequential dilution involves first diluting a sample with water or a replacement buffer to a
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