Transcription of DNA Fingerprinting
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DNA Fingerprinting
1 DNA Fingerprinting Unless they are identical twins, individuals haveunique DNA DNA Fingerprinting The name used for the unambiguous identifyingtechnique that takes advantage of differences in DNAsequence The process of DNA Fingerprinting begins byisolating DNA from blood, semen, vaginal fluids, hair roots, skin, skeletalremains, or elsewhere2 Polymerase Chain Reaction (PCR) If there is only a small amount of DNA available for DNAF ingerprinting augment the amount of DNA using a technique called PCR PCR is doing DNA replication in a test tube3 Polymerase Chain Reaction (PCR)Template Like ALL DNApolymerases Taq polymerase canonly add to the 3 endof an existingnucleotide A DNA primer that iscomplementary to thetemplate is used tosupply that 3 endand cool to anneal5 3 3 5 PrimerPrimerTemplate4 DNA Fingerprinting After we isolate the DNA and amplify it with PCR Treat the DNA with restriction enzymes cut DNA at specific sequences Everyone s DNA is different, so everyone s DNA will cut at differentsites This results in different sized fragments The different sized fragments are called restrictionfragment length polymorphisms, or RFLPs We can observe the different sized fragments in anexperiment that separates DNA based on fragment sizecalled Gel Electrophoresis5 RFLP Analysis Everyone has geneticsequences call
Cloning a Gene Using Bacteria Step 3. Insert the BGH Gene into the Bacterial Plasmid ¥The bacterial plasmid is also cut with the restriction enzyme, leaving sticky ends ÐA plasmid is a small circular DNA that is separate from the bacterial genome ¥Sticky ends of the cut BGH DNA attach by complementary base pairing to the sticky ends of the ...
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