Transcription of 木材腐朽菌定のために DNA バーコードとして 核の rDNA …
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木材腐朽菌定のために DNA バーコードとして 核の rDNA …
23 . DNA . rDNA ITS D1/D2 .. Use of ITS region and D1/D2 domain of nuclear rDNA as a two-locus DNA. barcode for identification of wood rot fungi.. 1 . 2 . 3 . 4 . 5 . 6 .. 1 .. DNA ITS DNA . DNA .. DNA ITS ITS . D1/D2 . D1/D2 PCR . 28 SrRNA D1/D2 600bp . ITS . D1/D2 . 23 55 . D1/D2 PCR . Neighbor Joining NJ .. DB D1/D2 .. PCR .. D1/D2 DB . D1/D2 . D1/D2 ITS .. PCR .. 2 .. RNA rRNA rDNA 1) . rDNA Internal transcribed spacer (ITS) ITS . DNA .. 5) . DNA DNA . DNA DNA . DNA DNA DNA .. rDNA .. rDNA . rDNA . rDNA .. rDNA NTS ( nontranscribed spacer ) ETS (external transcribed spacer ) 18S ITS ITS 28S 5S . 1 rDNA.. (60S ) 28S, , 5S. rRNA . (40S ) 18S rRNA . D1/D2 rDNA. (28S rRNA) . ITS rRNA 18S rRNA 28S. rRNA ITS ITS ITS 2) D1/D2 . 28S rRNA NL1 NL4 PCR.
3 実験方法 3-1 d1/d2 領域の塩基配列の決定 3-1-1 供試菌 木材腐朽菌の供試菌は木材住宅によく見られる23 種55 株(表3-1)の担子菌を試験に供した。
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