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Dr Shyamal Kr Paul

Dr Shyamal Kr Paul Associate Prof, Microbiology MMC. CULTURE MEDIA. 12/30/13 Dr. Shyamal Kr Paul, Culture media 1. Essential requirements in culture media Any culture medium must contains: -A source of energy -Sources of carbon, nitrogen, sulfur, phosphorus -Minerals, , Ca2+, Mg2+, Na+. -Vitamins and growth factors - Water 12/30/13 Dr. Shyamal Kr Paul, Culture media 2. Culture Media Culture Medium: Nutrients prepared for microbial growth Inoculum: Suspension of microorganisms Inoculation: Introduction of microbes into culture medium Culture: Microbes growing in/on culture medium A pure culture contains only one species or strain Mixed culture contains several species Contaminated culture contains unwanted species of organisms 12/30/13 Dr. Shyamal Kr Paul, Culture media 3. Culture Media pure 12/30/13 culture of Dr. Shyamal Kr Paul, Culture media mixed culture 4. Culture Media 12/30/13 contaminated culture Dr. Shyamal Kr Paul, Culture media 5. Culture Media Colony: visible growth of microbes on the surface of a solid medium A colony is a population of cells arising from a single cell or spore or from a group of attached cells A colony is often called a colony-forming unit (CFU).

Nutrient agar with 5% sheep blood Cultivation of fastidious and non fastidious bacteria. Differential – Identify hemolysis - Some bacteria secrete enzymes that lyse red blood cells (hemolysins) such that a clearing around the colony appears. βhemolysis- complete clearing (white hemolysis) αhemolysis – incomplete clearing (green hemolysis)

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