Transcription of Dr. Tim Sandle - Pharmig
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Dr. Tim Sandle Pharmaceutical Microbiology: Introduction What is environmental monitoring trying to do? Methods and limitations Problems and myths of incubation Which agar should you select? Should one or two agars be used? Which is the optimal temperature? For how long should you incubate for? What types of microorganisms can you expect to detect? Cleanroom microbiota Pharmaceutical Microbiology: Pharmaceutical Microbiology: Environmental monitoring To assess cleanrooms and controlled environments using viable and particulate counting methods. To verify environmental control (how well is the cleanroom working?). To assess impact of staff behaviours To help evaluate risk in relation to excursions (location and event dependent).
Optimal fungal medium It is generally accepted that TSA is a good, general medium for the recovery of bacteria. If two media are used, what is the appropriate fungal medium? Study: Gebala, B. and Sandle, T. (2013). Comparison of different fungal agar for the environmental monitoring of pharmaceutical-grade cleanrooms, PDA J Pharm Sci
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