Dr. Tim Sandle - Pharmig

Dr. Tim Sandle Pharmaceutical Microbiology: Introduction What is environmental monitoring trying to do? Methods and limitations Problems and myths of incubation Which agar should you select? Should one or two agars be used? Which is the optimal temperature? For how long should you incubate for? What types of microorganisms can you expect to detect? Cleanroom microbiota Pharmaceutical Microbiology: Pharmaceutical Microbiology: Environmental monitoring To assess cleanrooms and controlled environments using viable and particulate counting methods. To verify environmental control (how well is the cleanroom working?). To assess impact of staff behaviours To help evaluate risk in relation to excursions (location and event dependent). To assess event specific incidents HVAC losing power or maintenance works Pharmaceutical Microbiology: Limitations #1 EM methods The classic techniques: Active air-sampling: volumetric air-sampler Passive air-sampling: settle plates Surface samples: contact (RODAC) plates and swabs Personnel samples: Finger plates and gown plates New generation of real time' viable air samplers.

Tryptone soya agar (aka = soya-bean casein digest medium, tryptic soya agar)

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  Agar, Soya, Tryptone, Tryptone soya agar, Tim sandle, Sandle, Soya agar

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