Transcription of E. coli Gram Staining
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E. coli Gram Staining Written and Directed By: Colton Anderson Table of Contents iii Table of Contents Table of iii Introduction .. v Materials & Preparation .. 1 Materials and 3 Materials .. 3 Pre-Lab Preparation .. 3 Gram Stain Lab .. 5 Preparing the organism .. 7 Heat fixing .. 7 Gram Staining .. 8 Viewing Under the Microscope .. 11 Using the Microscope .. 12 Viewing your organism .. 12 Index .. 15 Introduction v Introduction It is important to be aware of the correct way to stain an organism. Without knowing the right way to do it you could potentially harm yourself and your lab partners. In this manual, you will be given direction on how to perform a simple gram stain of the E. coli bacteria. 3 Materials & Preparation In this section, we will gather all of the necessary materials to safely execute the lab procedure. 3 Materials and Preparation Materials 1 bunsen burner 1 Inoculating loop 1 Striker (to light the burner ) 1 Vial of E.
Also light your Bunsen burner at the beginning of the experiment. 5 . Gram Stain Lab. In the next section, we will go over how to prepare your organism and how to properly stain it. 7 Preparing the Organism . Heat fixing Heat fixing is a lab term in which the person doing the lab takes a
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