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Electrospray Ionization (ESI) - UMD

Electrospray Ionization (ESI) Electrospray Ionization (ESI)11 Electrospray is produced by applying a strong Electrospray is produced by applying a strong electric field to a liquid passing through a capillary electric field to a liquid passing through a capillary tube with a weak with a weak flux. Desolvation by gas flow (NDesolvation by gas flow (N) or gentle capillary ) or gentle capillary 22 Desolvation by gas flow (NDesolvation by gas flow (N22) or gentle capillary ) or gentle capillary heating (100heating (100--300 deg C).300 deg C). Ions are mostly preformed in solution before Ions are mostly preformed in solution before It is good for both small and large is good for both small and large molecules.

covalent, receptorcovalent, receptor-ligand complexes remain intact ligand complexes remain intact 22.. Working directly from a dilute soln: 0.001Working directly from a dilute soln: 0.001-10mM, 10mM, ... The X axis is mass divided by ... In negative ion mode the analysis is normally carried out well

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