Transcription of ELISA Technical Guide
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ELISA Technical Guide2 ELISA Technical GuideTable of contentsIntroduction ..3 ELISA technology ..4 ELISA components ..6 ELISA equipment ..7 Equipment maintenance and calibration ..8 Reagent handling and preparation ..9 Test component handling and preparation ..10 Quality control ..11 Sample handling ..12 Pipetting methods ..14 ELISA plate timing ..16 ELISA plate washing ..17 Plate reading and data management ..19 ELISA troubleshooting ..20 Appendix A: Gravimetric pipette calibration procedure ..24 Appendix B: Inventory control tracking chart ..25 Appendix C: Laboratory tracking chart ..26 Appendix D: Maintenance and calibration schedule.
An ELISA is a set of standardized reagents and microwell plates manufactured for a specific test. An IDEXX ELISA may contain some or all of the following components: coated plates (solid and/or strip plates), sample diluent, controls, wash concentrate, conjugate, substrate, and stop solution. The tests are manufactured in batches or lots.
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