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ELISA Technical Guide

ELISA Technical Guide2 ELISA Technical GuideTable of contentsIntroduction ..3 ELISA technology ..4 ELISA components ..6 ELISA equipment ..7 Equipment maintenance and calibration ..8 Reagent handling and preparation ..9 Test component handling and preparation ..10 Quality control ..11 Sample handling ..12 Pipetting methods ..14 ELISA plate timing ..16 ELISA plate washing ..17 Plate reading and data management ..19 ELISA troubleshooting ..20 Appendix A: Gravimetric pipette calibration procedure ..24 Appendix B: Inventory control tracking chart ..25 Appendix C: Laboratory tracking chart ..26 Appendix D: Maintenance and calibration schedule.

The enzyme-linked immunosorbent assay (ELISA) is one of the most sensitive and reproducible technologies available. These assays are rapid, simple to perform, and easily automated. IDEXX introduced the first commercial poultry ELISA for infectious bursal disease (IBD) in 1985 and the first commercial livestock ELISA for Aujeszky’s

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  Assay, Enzymes, Linked, Idexx, Immunosorbent, Linked immunosorbent assay

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