Transcription of ELISA Technical Guide - IDEXX US
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ELISA Technical Guide2 ELISA Technical GuideTable of contentsIntroduction ..3 ELISA technology ..4 ELISA components ..6 ELISA equipment ..7 Equipment maintenance and calibration ..8 Reagent handling and preparation ..9 Test component handling and preparation ..10 Quality control ..11 Sample handling ..12 Pipetting methods ..14 ELISA plate timing ..16 ELISA plate washing ..17 Plate reading and data management ..19 ELISA troubleshooting ..20 Appendix A: Gravimetric pipette calibration procedure ..24 Appendix B: Inventory control tracking chart ..25 Appendix C: Laboratory tracking chart ..26 Appendix D: Maintenance and calibration schedule ..27 Appendix E: Quality control quick check ..28 Notes ..293 ELISA Technical GuideIntroductionIDEXX manufactures diagnostic tests for the detection of diseases in ruminants, equines, swine, and enzyme-linked immunosorbent assay ( ELISA ) is one of the most sensitive and reproducible technologies available.
7 ELISA Technical Guide ELISA equipment Equipment for ELISA testing is widely available. Readers, washers, and pipettes are available as manual or automated systems. Some of the factors affecting equipment selection are the number and types of tests and samples, technical training of staff, and financial considerations. Below is a brief outline
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