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Examples of Image Analysis Using ImageJ

Examples of Image Analysis Using ImageJ . Area Measurements of a Complex Object Problem: Determine the photosynthetic ( , green) portion of a variegated leaf. (Open leaf Image via Select File Open Samples Leaf). Convert scanned color Image of leaf to grayscale: Image Type 8-bit Set measurement scale: Draw a line over a 50 mm section of the ruler then Analyze Set Scale In Set Scale window enter 50 into the 'Known Distance' box and change the 'Unit of Measurement' box to mm , check 'Global'. Draw a new line and confirm that the measurement scale is correct. Threshold the leaf Image Using the automated routine: Process Binary Make Binary The automated threshold includes only the dark green areas. Calculate area of green portion: Surround the leaf with the rectangular selection tool Analyze Analyze Particles Enter 50 as the minimum particle size, toggle 'Show Outlines', check Display Results' and click 'OK'.

Problem: Determine the photosynthetic (i.e., green) portion of a variegated leaf. (Open leaf image via Select File → Open Samples → Leaf) Convert scanned color image of leaf to grayscale: Image → Type → 8-bit Set measurement scale: Draw a line over a 50 mm section of the ruler then Analyze → Set Scale

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Automated Segmentation and Morphometry of Cell and …

imagej.nih.gov

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ImageJ User Guide - National Institutes of Health

imagej.nih.gov

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imagej.nih.gov

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imagej.nih.gov

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imagej.nih.gov

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imagej.nih.gov

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imagej.nih.gov

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extension.cropsciences.illinois.edu

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oceanexplorer.noaa.gov

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secure-media.collegeboard.org

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ncert.nic.in

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