Transcription of EXERCISE 8 & 9 Laboratory Procedure Manual …
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EXERCISE 8 & 9 Laboratory Procedure Manual …
EXERCISE 8 & 9 Laboratory Procedure ManualMLAB 2431 86 DAT and ElutionExercise 8 direct Antiglobulin Test (DAT) EXERCISE 9 Elution StudyTaskTo perform the DAT and elution Procedure with correct interpretation of results. AimTo perform with 100% accuracy the DAT Procedure and elution Procedure with correctinterpretation of results. IntroductionDATThe direct antiglobulin test (DAT) is used to detect in vivo red blood cell red blood cells from the patient are directly tested with antiglobulin serum. Thespecimen of choice for the DAT is an EDTA specimen. If a clotted specimen is used afalse positive result may be obtained due to non-specific binding of complement to the redblood cells in-vitro. EDTA binds calcium which is necessary for complement focus of this lab is to determine sensitization of baby s red blood cells, but the DATis a Procedure which may also be performed on other types of patients as well as donorunits. The DAT is utilized in the blood bank to aid in the diagnosis of a variety ofconditions such of hemolytic disease of the newborn (HDN).
EXERCISE 8 & 9 Laboratory Procedure Manual DAT and Elution MLAB 2431 •86 Exercise 8 Direct Antiglobulin Test (DAT) Exercise 9 Elution Study Task To p erf orm t h DAT a nd e lu tiroce re w c rrec i rp a of re s. Aim T o perf rm wi t h 100% accuracy t e DA pro cedure a nd el ut i pr oc edur w co rr ect interpretation of results. Introduction DAT The direct …
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