Transcription of EXPERIMENT 4: COLUMN CHROMATOGRAPHY AND THIN …
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EXPERIMENT 4: COLUMN CHROMATOGRAPHY AND THIN …
EXPERIMENT 4: COLUMN CHROMATOGRAPHY AND THIN LAYER CHROMATOGRAPHY THE SEPARATION, ISOLATION AND ANALYSIS OF THE DIFFERENT COMPONENTS IN A MIXTURE. GRAVITY COLUMN CHROMATOGRAPHY USED TO SEPARATE (AND THUS PURIFY) THE COMPONENTS OF A MIXTURE (PAPRIKA PIGMENTS). SEPARATION IS ACHIEVED BY PARTITIONING THE COMPONENTS BETWEEN A STATIONARY PHASE AND A MOBILE PHASE. STATIONARY PHASE MOBILE PHASE SILICA GEL ORGANIC SOLVENTS COLUMN CHROMATOGRAPHY : PREPARATION HALF FILL THE COLUMN WITH HEXANE, THEN ADD ~ 4g OF SILICA GEL WHILST GENTLY TAPPING THE COLUMN .
perform column chromatography in the fume hood, tlc on the bench. to save time, run the tlc experiment while the column chromatography is underway. keep an eye on solvent labels and dispose of all waste in the appropriate containers. no lab report is required for experiment 4. there will be a quiz on experiments 3 & 4 next week.
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