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Experimental design and sample size determination

1 Experimental designand sample size determinationKarl W BromanDepartment of BiostatisticsJohns Hopkins ~kbromanNote This is a shortened version of a lecture which is part of a web-based course on Enhancing Humane Science/ImprovingAnimal Research (organized by Alan Goldberg, Johns HopkinsCenter for Alternatives to Animal Testing) Few details mostly design3 Basic question/goal in (aka blocking) experiments43 ExampleQuestion:Does salted drinking water affect blood pressure (BP) in mice? a mouse with water containing 1% 14 experiments are comparative. Compare BP in mice fed salt water to BP in micefed plain water.

in strain B mice fed salt water. Ideally, the experimental group is compared to concurrent controls (rather than to historical controls). ... •Reduce the effect of uncontrolled variation (i.e., increase precision). •Quantify uncertainty. A related point: An estimate is of no value without some statement of the uncertainty in the estimate. 8 ...

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