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Quenching of fluorescence is the basis for many investigations using fluorescence. The introduction provided in the following is also instructive of basic first and second order kinetics, which you will be studying in Lecture. We will use the equations and data from the Theory handout.

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Introduction to Fluorescence Spectroscopies I. Theory

www.chemistry.montana.edu

fluorescence intensity emitted by the fluorophore because of electron transfer to or from another molecule (called the quencher) to or from the fluorescing molecule, or transfer of the fluorophore’s excitation energy to another molecule.

  Introduction, Fluorescence, Introduction to fluorescence

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wiki.utep.edu

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